|
M. catarrhalis
|
|
|
| O35E |
WT isolate from middle ear effusion (Dallas, TX) |
[82]
|
| O35E.B1B2 |
mhaB1mhaB2 double isogenic mutant of strain O35E,spectinomycin and zeocin resistant |
[67]
|
| O35E. TN2 |
hag transposon mutant of strain O35E, kanamycin resistant |
[132]
|
| O35E.2 |
uspA2 isogenic mutant of strain O35E, kanamycin resistant |
[133]
|
| O35E.CD1 |
ompCD isogenic mutant of strain O35E, kanamycin resistant |
[72]
|
| O12E |
WT isolate from middle ear effusion (Dallas, TX) |
[66]
|
| McGHS1 |
WT isolate from patient with respiratory infection (Toledo, OH) |
[70]
|
|
E. coli
|
|
|
| EPI300™ |
Cloning strain for recombinant DNA methods |
Epicentre® (Illumina®) |
| TUNER™ |
Expression strain for protein purification purposes |
EMD Millipore |
|
Plasmids
|
|
|
| pGEX4T-2 |
Protein expression vector, ampicillin resistant |
GE Healthcare Life Sciences |
| pGEX-MhaB |
pGEX4T-2 expressing O35E MhaB1 aa 72–399 joined to aGST N-terminal tag (GST-MhaB), ampicillin resistant |
This study |
| pGEX-McaP |
pGEX4T-2 expressing O35E McaP aa 51–333 joined to aGST N-terminal tag (GST-McaP), ampicillin resistant |
This study |
| pRBHis.MhaB.72.399 |
pETcoco-1 expressing O12E MhaB1 aa 72–399 joined to6 N-terminal histidine residues (His-MhaB), chloramphenicol resistant |
[67]
|
