Figure 5. Alex3 degradation is independent of the canonical Wnt/β-catenin pathway.

(A,B) Constitutively active β-catenin (red) neither induces Alex3 protein degradation, as seen in WB (A), nor reverts the aggregated mitochondrial phenotypes induced by Alex3 overexpression (green in B). Nuclei were visualized in blue (bisbenzimide) (B). (C,D) Neither co-transfection with Dvl2 (C) nor the inhibition of GSK3β with 10 mM LiCl or with 10 µM SB212763 (D) induces Alex3 protein degradation. Wnt1 transfection was used as a control for Alex3 degradation. Scale bar: 10 µm.