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. 2013 Jul 2;11(7):e1001599. doi: 10.1371/journal.pbio.1001599

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© 2013 Andrews et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) ³¹P NMR spectra of P_i bound to S102G/R166S AP at various pH values. See Materials and Methods for conditions. (B) The chemical shift of P_i-bound to S102G/R166S AP versus the solution pH. A nonlinear least-squares fit to an equation (see Materials and Methods) derived from the binding model in (C) yields δ_upfield and δ_downfield values of −0.74 and 1.94 ppm, respectively, and a value for of 10^−6.1 M as defined in (D).

Inline graphic — (A) ³¹P NMR spectra of P_i bound to S102G/R166S AP at various pH values. See Materials and Methods for conditions. (B) The chemical shift of P_i-bound to S102G/R166S AP versus the solution pH. A nonlinear least-squares fit to an equation (see Materials and Methods) derived from the binding model in (C) yields δ_upfield and δ_downfield values of −0.74 and 1.94 ppm, respectively, and a value for of 10^−6.1 M as defined in (D).