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. 2013 Jul 2;8(7):e67748. doi: 10.1371/journal.pone.0067748

Figure 4. DNA demethylating agents but not histone acetylation inhibition revert the IFN-γ inhibitory effect.

Figure 4

C57BL/6 mice received 2.5×1011 vg/kg of AAV8-luc vector alone or in combination with 1.5×109 vg/kg of AAV8-IL12 vector. One group of 5 mice treated with AAV8-luc or AAV8-luc in combination with AAV8-IL12 received 5′-Azacytidine (AZA) intraperitoneally at a dose of 1 mg/kg every 24 hours starting the day of vector injection. A second group of 5 mice treated with AAV8-luc or AAV8-luc in combination with AAV8-IL12 received 2 mg/kg of trichostatin A (TSA) were injected IP every 48 hours starting the day of vector injection. A third group of animals received only vector injection. Luciferase expression levels were analyzed in both groups of animals at days 1, 4, and 7 using a Xenogen in vivo luminometer, experiments could not be performed at longer time points due to the toxicity of 5-AZA long term treatment. Luciferase expression was quantified and represented as photons/second. The data are shown as mean ± standard deviation (SD). The differences in luciferase expression were statistically evaluated by Student t test (**p<0.01).