Figure 6.

Cancer cachexia decreases the phosphorylation of S6 ribosomal protein and 4EBP1 in the heart. Immunoblot analysis for total and phosphorylated S6rp at Ser235/236 in BL6 and Apc^Min/+ mice of 12 and 20 weeks (A and B). Total and phosphorylated levels of 4EBP1 at Thr 37/46 in BL6 and Apc^Min/+ mice at 12 and 20 weeks (C and D). Data are normalized relative to the amount of GAPDH and results are expressed as the ratio of phosphorylated to total levels of S6rp and 4EBP1, respectively, in arbitrary units for comparison. An asterisk (*) indicates significant differences in Apc^Min/+ mice from the BL6 age-matched controls (n=4–6 per group, P<0.05).