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A) Protein purity assessed on SDS-PAGE. MW: molecular weight standard from Euromedex; 1: Cfp21 WT; 2: Cfp21-A1; 3: Cfp21-A2; 4: Cfp21-A3; 5: Cfp21-A1A2; 6: Cfp21-A2A3; 7: Cfp21-A1A3; 8: Cfp21-TM. 1 µg of each protein was loaded onto a 15% polyacrylamide gel and stained with Coomassie Blue. B) Far UV spectra of the Cfp21 mutant proteins.
