Table 1. Excitation distribution intensity profiles.
| Penetrationdepth (µm) | 1PE Low scattering (5 mm−1) | 1PE High scattering (50 mm−1) | 2PE Low scattering (5 mm−1) | 2PE High scattering (50 mm−1) |
| 400 µm | 8.2±0.6 µm | 12.6±0.8 µm R2 = 0.97 | 7.2±0.4 µm | 8.2±0.5 µm R2 = 0.98 |
| 600 µm | 8.6±0.5 µm | 20.7±1.3 µm R2 = 0.99 | 7.6±0.6 µm | 9.2±0.8 µm R2 = 0.99 |
| 800 µm | 9.6±0.6 µm | 21.9±1.0 µm R2 = 0.99 | 8.5±0.9 µm | 10.2±1.0 µm R2 = 0.98 |
Summary of mean values of the FWHM of light-sheet intensity profiles along the axial path in single- and two-photon excitation while increasing the illumination path. A greater thickness of the effective excitation distribution is observed for single-photon excitation (488 nm) compared with two-photon excitation (790 nm) when the light is focused in a highly scattering (50 mm−1) samples (the mean adjusted R2 is indicated). For measurements performed in the low scattering regime the adjusted R2 values are higher than 0.99. The imaging depth is set at 150 µm.