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. 2013 Jul 2;8(7):e67735. doi: 10.1371/journal.pone.0067735

Figure 1. Replication of X4 virus in DC-T cell co-cultures is influenced by the nature of PAMPs.

Figure 1

iDCs were first either left untreated or treated for 24 hours with the listed PAMPs. Next, cells were exposed to X4-using NL4-3 for 1 hour at 37°C before initiation of a co-culture with autologous resting CD4+ T cells. Cell-free supernatants were harvested at 3, 6 and 9 days after initiation of the co-culture and the viral content was assessed by performing a p24 ELISA test. (A) Data shown represent the means ± SEM of quadruplicate samples from one representative donor out of six at day 6 after initiation of the co-culture (days post-coculture/dpcc). The small insert shows kinetics of virus production for this representative donor (only iDCs either left untreated or treated with flagellin or zymosan are illustrated). (B) This panel illustrates data for all six independent donors. Each point represents the mean of quadruplicate samples for each donor. The horizontal line represents median results of all donors. Asterisks denote statistically significant data (*: p<0.05; **: p<0.01).