Figure 6. Replication of X4 virus in DC-T cell co-cultures in presence of different PAMPs and AMD3100.

iDCs were first either left untreated or treated for 24 hours with the listed PAMPs. Next, cell were extensively washed 3 times with PBS and treated for 30 min with AMD3100 (20 µg/ml), washed again extensively to remove the residual drug and inoculated with NL4-3 (10 ng of p24 per 10⁵ cells) for 1 hour at 37°C. Cells were again extensively washed before initiation of co-culture with autologous resting CD4⁺ T cells. Cell-free supernatants were harvested at different days following initiation of the co-culture (day 10 is depicted). The viral content was assessed by performing a p24 ELISA test. Data shown represent the means ± SEM of quadruplicate samples from two distinct donors. Asterisks denote statistically significant data (**: p<0.01). The small insert shows kinetics of virus production for one donor.