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. 2013 Jul 2;8(7):e68487. doi: 10.1371/journal.pone.0068487

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© 2013 Dantas et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Bar diagram of chicken centrin2, showing the relative positions of the phospho-sites and EF-hand residues that were mutated in this study.

B. Immunoblot showing the relative expression levels of the myc-centrin2 transgenes in the clones used in this study. Numerical values show the mean of 3 separate experiments.

C. Immunofluorescence micrograph showing the localisation of wild-type and the indicated mutants of centrin2 after stable transfection of Cetn4 ^-/- /2 ^-/- /3 ^- DT40 cells. DNA was labelled with DAPI (blue). Scale bar, 5 µm.

D. Quantitation of the relative centrin2 signal detected at the centrosome (co-localisation with γ-tubulin). Histogram shows the mean percentage + s.d. of the control signal seen in at least 1000 cells in 3 separate experiments. The control was Cetn4 ^-/- /2 ^-/- /3 ^- DT40 cells that stably expressed wild-type myc-centrin2. *, P <0.05; **, P <0.01 compared to controls by Kruskal-Wallis test and Dunn’s multiple comparison test. Size markers are indicated at right.