Figure 3. Centrin deficiency does not impact on protein levels of the XPC-RAD23 complex.
A. Immunoblot shows the expression level of centrin2 in cells of the indicated genotype. Two bands are seen in the myc-centrin2 rescue clone due to a second methionine in the centrin2 transgene coding sequence serving as a second potential start site.
Immunoblots show the levels of B. RAD23A and RAD23B; C. XPC before and at the indicated times after 20J/m2 UV irradiation in wild-type, centrin-deficient and rescued DT40 cells. The rescue clone was that used in Figure 1B. Where indicated, cycloheximide (CHX) was added at 100µg/ml at time 0. Results shown are representative of 3 experiments. α- and γ-Tubulin were used as loading controls. Size markers are indicated at right.