Skip to main content
. 2013 Jul 2;8(7):e68487. doi: 10.1371/journal.pone.0068487

Figure 5. cPOC5 overexpression induces linear structures.

Figure 5

A. Immunofluorescence micrograph shows examples of the structures induced by transient transfection of DT40 cells with a cPOC5-GFP expression construct. GFP is shown in the green channel, centrosomes were identified by γ-tubulin staining (red) and DNA was visualised with DAPI (blue). Micrographs are representative of results obtained in at least 2 separate experiments. Scale bar, 5 µm.

B. Centrin colocalisation with cPOC5-GFP structures was observed by microscopy for centrin2 (red), with cells otherwise being transfected, fixed and stained as for A.

C. Centrosomal localisation of cPOC5-GFP was observed in centrin-deficient cells. Cells were transfected, fixed and stained as for A.