Figure 5. In vivo inhibition of transcription in C. albicans by MMGP1.

(a) Confocal micrographs showing inhibition of transcription in C. albicans by MMGP1. The images are overlay of TMR-florescent azide (red), Hoechst 33342 (blue) and bright field micrographs of C. albicans cells. Intense EU staining (red fluorescence) was observed in nucleus after 2 h of treatment with MMGP1 and prolonged treatment of cells with peptide showed decrease in EU signal in the nucleus (b) Quantification of transcription inhibition in MMGP1-treated C. albicans by flow cytometry (X²-C. albicans cells showing TMR-A fluorescence i.e cells that are transcriptionally active).