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. 2013 Jul 2;105(1):101–107. doi: 10.1016/j.bpj.2013.04.022

Figure 3.

Figure 3

Protons slow the onset of gating charge immobilization in NaV1.5. (A) To assess the onset of charge immobilization, immobilized charge was normalized to the total QOFF of each sweep, plotted as a function of time, and fitted with a single exponential function (Eq. 3) for pH 7.4 (solid squares) and pH 6.0 (open squares). pH 6.0 significantly increased the time constant (τ) of charge immobilization onset from 1.1 ± 0.3 ms to 1.5 ± 0.4 ms and reduced the total charge immobilized from 70.5 ± 5.7 to 62.4 ± 6.1% (n = 6, p < 0.05). (Insets) Pulse protocols used. (B) Normalized QON is plotted as a function of time and fitted with a double exponential function (Eq. 2). We observed no statistically significant effect on the recovery of gating charge at pH 6.0 (open squares) compared to pH 7.4 (solid squares) (n = 5).