Figure 2.

Antigen-specific IgG₁ titers change over time. In all graphs, shown are means ± SEM. (A) Mice that underwent IRI versus sham surgery and then had ip immunization with 25 μg NP-keyhole limpet hemocyanin (KLH) 24 hours after surgery had sustained increases in NP-specific IgG₁ titers (P=0.04 by mixed model analysis beginning at day 35). Data shown are from ELISA; n=5 for IRI-treated group and n=4 for sham-treated group. (B) The effect of reperfusion time on the immune response was tested. Although the mouse is in the recovery phase from IRI at 4 days, the augmentation effect was greatest at that time. Data shown are from day 35 after immunization. Data shown are from ELISA; n=5 for day −7 IRI, n=6 for day −4 IRI, n=5 for day −1 IRI, and n=3 for sham-treated. By ANOVA, P=0.10.