Figure 6.
Mice that cannot produce IL-10 do not display the observed effect after IRI. (A) Mice with a targeted genetic deletion of the gene for IL-10 were subjected to renal IRI or sham treatment, and they were immunized with NP-KLH after 24 hours of reperfusion. The IL-10−/− mice subjected to IRI did not show increased titers of anti-NP IgG1 compared with sham-treated animals. Data shown are from ELISA; n=3 for IRI-treated group and n=3 for sham-treated group. (B) qPCR data from IRI- versus sham-treated kidneys show that WT mice produce IL-10 within the kidney after renal IRI. The fB−/− mice produced significantly lower amounts of IL-10 after renal IRI than wild-type animals. Data are reported relative to WT sham-treated kidneys. Primer t test P=0.10.