Figure 6.

Western blot analysis of the levels of various signaling pathway intermediates relevant to cell proliferation and apoptosis in E12.5 kidneys. (A) Phospho(p)-p38 MAPK levels were significantly reduced in the double mutants compared with controls. SIX2 levels were similar or slightly reduced in double mutants compared with controls after normalization to actin. (B and C) Levels of p-ERK, ERK, p-JNK, JNK, p-c-Raf, and c-Raf were reduced in the double mutants compared with control, with p-JNK and p-ERK being more reduced than total JNK and ERK. (D) Levels of p-AKT and AKT were similar or slightly reduced in the double mutants versus controls.