Figure 9. AmTrac1;2 and MepTrac response.

(A) Growth complementation of Δmep1,2,3 yeast expressing MEP2, MepTrac, their H194E mutants or AmTrac1;2 on solid media containing 2 mM NH₄Cl or 1 mM arginine (growth control) as sole nitrogen source for 3 days. (B) Fluorescence response of Δmep1,2,3 yeast expressing AmTrac1;2, MepTrac or MepTrac-H194E to NH₄Cl at the indicated concentrations at pH 4 or 6. AmTrac1;2 was tested at pH 6. Data are normalized to water-treated control (0) (mean ± SE; n = 3; SNK test: *p<0.01).

DOI: http://dx.doi.org/10.7554/eLife.00800.012