Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Peptides are separated using HPLC and analyzed using two mass analyzers. In the first quadrupole mass analyzer, the intact peptide is selected and then fragmented using collision-induced dissociation in the collision cell and fragments are selected in the last quadrupole. Any ions that strike the detector have the correct m/z of the intact precursor and the correct fragment m/z.
