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. 2013 Mar 6;22(14):2047–2056. doi: 10.1089/scd.2012.0615

FIG. 5.

FIG. 5.

Histological observation of bone reconstruction in the BMMSC-treated BRONJ minipig model. (A) H&E staining showed new bone formation in the NB area 12 weeks and 12 months after BMMSC infusion (scale bar=300 μm), and NB was still present in minipigs with BRONJ treated with saline (scale bar=300 μm). (B) Positive staining of Y-chromosomes (red) in regenerated bone of the BRONJ region (yellow arrowhead). (C–F) Bone remodeling of the mandible in minipigs after treatment with BMMSCs. Osteoclasts (C, black arrowhead) and osteoblasts (E, green arrowhead) were observed in H&E-stained sections 12 weeks after BMMSC infusions (bar=50 μm). Means of osteoclast/field (D) and osteoblast/field (F) in H&E-stained sections were also significantly higher in the BMMSC-treated group **P<0.01. (G) Trichrome staining revealed new bone formation (osteoid, red staining) in the collagen matrix (scale bar=300 μm) of the group treated with BMMSCs 12 months postinfusion, whereas minipigs with BRONJ treated with saline showed no marked new bone formation (scale bar=300 μm). (H) TRAP staining showed that the osteoclasts (black arrowhead) were observed in the group treated with BMMSCs 12 months postinfusion, whereas minipigs with BRONJ treated with saline showed few osteoclasts (bar=50 μm). A significant increase in the osteoid area (I) and osteoclasts (J) over the tissue area in the group treated with BMMSCs compared with the group treated with saline (***P<0.001).