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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Pain. 2013 Mar 7;154(8):1170–1180. doi: 10.1016/j.pain.2013.02.027

Figure 5.

Figure 5

Effects of NaV1.6 knockdown after DRG inflammation. NaV1.6 siRNA or nontargeting siRNA was injected into the DRG at the time of DRG inflammation (POD0). Baseline values for ipsilateral paw withdrawal thresholds to mechanical stimuli (A) and the percent of animals withdrawing the paw in response to light stroking with a cotton wisp (B) were measured on 2 – 3 different days prior to surgery and the averages are plotted on POD0. A: prolonged mechanical hypersensitivity was observed in nontargeting siRNA treated animals (n = 8). This was completely abrogated in NaV1.6 siRNA injected animals (n = 10). ***, difference between the 2 groups was significant (p<0.001)on each POD (except baseline), 2-way RM ANOVA with Bonferroni posttest). B: percent responding to cotton wisp test. No animals responded prior to DRG inflammation on POD0. **, significant difference between groups on POD1 to POD 21, p<0.01 (except POD 3, p<0.001; and POD10, p<0.05), Fisher’s exact test. C, D: examples of DRG sections from nontargeting (C) or NaV1.6 siRNA (D) treated inflamed DRG on POD3 stained for NaV1.6. Scale bar=50 μm.