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A. Binding measured by fluorescence anisotropy of the complex of full-length PXR with full-length RXRα to a FAM-labeled 15-bp DNA duplex composed of a DR3 motif from the promoter of the human CYP3A4 gene product (see Tables 3, 4). B. Competition binding experiments using fluorescence anisotropy and monitoring the dissociation of the FAM-DR3 DNA duplex by increasing concentrations of unlabeled duplexes (see Tables 3, 4).
