FIG. 1.

Complete re-endothelialization of the aortic valve scaffold after cultivation in a dynamic bioreactor. Hematoxylin and eosin and immunohistochemical images show complete coverage by cells expressing eNOS (red) and CD31 (green) on the surface of the aortic wall (A, E), the edge of the leaflet (B, F), and the sinus of the valve (C, G). Cell nuclei positive for DAPI. (Bars 200 μm) Phalloidin stain shows homogenous cell distribution over the whole leaflet surface (D, H). (Scale bars 50 μm). eNOS, endothelial nitric oxide synthase. Color images available online at www.liebertpub.com/tea