FIG. 5.

Immunhistochemical micrographs from explants. Massive leukocyte infiltration of the CPV wall (A) and leaflet (B) with CD45 cells (red). The preserved endothelial cell layer over the CPV wall (C) positive for CD31/eNOS (green/red) and cusp (D)-positive for vWF (green). The TEV wall (E) and leaflet (F) without leukocyte infiltration (red—classified as background staining in the absence of concomitant staining of cell nuclei in blue). The TEV cusp (G) covered by CD31/eNOS (green/red)-positive cells. Expression of vWF by cells over the TEV wall (H). (Scale bars 200 μm). vWF, von Willebrand factor. Color images available online at www.liebertpub.com/tea