FIG. 3.

Developmental potential of hESC-derived mesenchymal cells in vivo. hESC-derived mesenchymal cells were seeded on the PLGA/PLLA scaffolds or HA-composite PLGA/PLLA scaffolds and a predifferentiated in osteogenic condition for 10 days in vitro before implantation. (A) Gross image of in vivo engineered tissues after 8 weeks of implantation. (B) H&E staining of the implanted scaffolds after 4 and 8 weeks. (C) Quantification of blood vessels normalized to the unit area after 4 and 8 weeks of implantation. (D) Blood vessel networks were evident throughout the engineered tissue in the HA-0 scaffold. Arrow denotes red blood cells. Immunostaining using human specific anti-CD31 (E) and anti-VE-Cadherin (F) taken from the HA-0 scaffold. HA-0: PLGA/PLLA scaffold, HA-1: PLGA/PLLA scaffold with 1% w/v HA; HA-5: PLGA/PLLA scaffold with 5% w/v HA. Scale bar, 50 μm. *p<0.05. Color images available online at www.liebertpub.com/tea