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Biomaterial-directed osteogenic differentiations of hESC-derived mesenchymal cells in vivo. (A) Cells seeded on the PLGA/PLLA scaffold show positive for Alcian blue staining for negatively charged proteoglycans, and while 5% HA–PLGA/PLLA composite scaffolds show direct bone formation by Alizarin red mineral staining after 4 weeks of implantation. (B) Quantification of cartilaginous area. Scale bar, 100 μm. **p<0.01. Color images available online at www.liebertpub.com/tea
