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. Author manuscript; available in PMC: 2014 Jul 24.
Published in final edited form as: J Mol Biol. 2013 Apr 23;425(14):2415–2422. doi: 10.1016/j.jmb.2013.04.016

Figure 1.

Figure 1

Reconstitution of Sec incorporation in wheat germ in vitro translation lysate. In vitro translation of the Sec incorporation reporter mRNA (A) in 50 % of wheat germ lysate in the presence or absence of 160 nM XH-SBP2 and FLAG-eEFSec recombinant proteins, 1.25 μg of total testes aminoacylated tRNA and 80 nM of salt washed rabbit ribosomes (B). Data was normalized for luciferase activity from mutant GPX4 SECIS element. The data represents the mean and standard deviation of three independent experiments (n=3). The asterisk (*) denotes a significant difference vs. no factors by student’s t-test (p < 0.02). (C) In vitro translation of a Sec incorporation reporter mRNA that has a UAA codon instead of the UGA codon shown in (A). Raw luciferase activity (luminescence) was measured by luminometry. The double asterisk (**) denotes a significant difference vs. no SBP2 by student’s t-test (p < 0.02).