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Mammalian ribosomes are required for Sec incorporation. Total translation (A) or Sec incorporation (B) in ribosome-depleted RRL in the presence of 80 nM salt washed ribosomes from rabbit reticulocyte lysate, Saccharomyces cerevisiae, Spodoptera frugiperda Sf21 cells or wheat germ lysate as indicated. The asterisk (*) denotes a significant difference vs. no ribosomes by student’s t-test (p < 0.02).
