Fig 5.

The PLCγ-RasGRP1 axis contributes only minimally to p38 activation in thymocytes. (A and B) Pharmacological inhibition of DAG-producing PLCγ by U73122 results in a modest reduction of p38 activation compared to the reduction in ERK activation in TCR-stimulated total thymocytes and Jurkat cells. U73343, inert analog, is used as a treatment control. Percentages relative to the control maximum activation are shown. Representative of two experiments. (C) TCR-stimulated MKK3/6 and p38 activation is compared between wild-type and LAT-deficient J.Cam2 Jurkat cells. (D and E) Minute reduction in TCR-induced p38 activation in Rasgrp1-deficient thymocytes. Control Rasgrp1^+/+ thymocytes were obtained from MHCI/II double-deficient mice. Data are means ± SD from at least three mice of each genotype (Student t test; *, P < 0.05; **, P < 0.01).