FIG. 3.
Density properties of lung progenitor cell lineages. Sham or 2-day postbleomycin-treated mice were injected with BrdU, euthanized, and lungs harvested after 1 h. Cells were then dissociated, colabeled for BrdU and CD45, SCA-1, c-KIT, CD31, EpCAM, or CD49f, and processed for flow cytometry. (A) In untreated mice, BrdU-incorporating cells of FR3, primarily belonged to EpCAM and CD49f cell lineages. In contrast, BrdU-incorporating cells of fraction FR4 were SCA-1-positive (n≥5). (B) Bleomycin treatment diminished the presence of BrdU-incorporating EpCAM and CD49f putative progenitor cells in fraction 3 introducing, instead, proliferating CD45, SCA-1, and c-KIT cell lineages to fraction 5 (n≥4). Data are presented as average±SEM. (C) Representative flow cytometry analysis of CD49f and EpCAM expression demonstrating the gating and percentage of sorted CD49f and EpCAMhi cells from density fractions.