Table 1.
Properties of the Studied Density Fractions and Eluted Cells from the Homeostatic Lung
| Fraction | Average density (g/cm3)a | % of total lung cellsa | Nucleus–cell ratioa | Composite cell types of the homeostatic lung |
|---|---|---|---|---|
| FR1 | 1.025±0.001 | 9.3±5.2 | 0.82 | AT1, endothelial |
| FR2 | 1.029±0.001 | 15.6±7.0 | 0.59 | AT1, endothelial |
| FR3 | 1.039±0.002 | 11.4±1.7 | 0.57 | AT2 and secretory (epithelial progenitors), myofibroblast, endothelial |
| FR4 | 1.058±0.003 | 30.3±4.7 | 0.58 | AT2, secretory, myofibroblast, endothelial |
| FR5 | 1.074±0.005 | 33.3±8.4 | 0.55 | AT2, secretory, endothelial |
Distribution of total mouse cell homogenate by density shows a majority of lung-extracted cells equilibrate at the higher densities. The nucleus to cell ratio of cells from fraction 1 significantly differs from all other fractions. Composite cell-type classification is based upon transcript and protein expression of lineage-specific markers. Average density data are also found in Fig. 1B. Nucleus and cell ratios are found in Supplementary Fig. 1. Data are presented as average±SEM. aP<0.001.