Table 2.
Enzymatic activities of wild-type and mutant mitochondria
| Strain | Enzymatic activitya |
||
|---|---|---|---|
| NADH:HAR (arbitrary units) | NADH oxidaseb (nmol O2 min−1 mg−1) (% rotenone sensitivity) | Complex I (nmol O2 min−1 mg−1) (% of wt) | |
| Wild type | 0.434 ± 0.036 | 146.9 ± 14.0 (64) | 94.0 (100) |
| nuo13.4 | 0.334 ± 0.020 | 232.8 ± 20.2 (32) | 74.5 (79.2) |
| 13.4L | 0.360 ± 0.034 | 135.6 ± 14.4 (50) | 67.8 (72.1) |
| nuo13.4×L | 0.226 ± 0.016 | 190.2 ± 20.7 (21) | 39.9 (42.4) |
a
Values are means and standard errors from at least 4 independent mitochondrial preparations.
b
Activity was measured in the presence of 20 μg/ml alamethicin. Respiratory activities were fully sensitive to 0.4 μM antimycin A.