Fig 7.

Net1A is specifically required for invasion of a Matrigel extracellular matrix. (A) MDA-MB-231 cells were transfected with control- or Net1 dual isoform-specific siRNAs and then allowed to invade overnight through a Matrigel-coated Transwell apparatus. Both wells contained 10% FBS. The inset shows a representative Western blot using an antibody that primarily recognizes Net1. Shown is the average from four independent experiments. Errors are SEM. **, P < 0.01. (B) MDA-MB-231 cells were transfected with the siRNAs indicated. Two days later, the cells were serum starved and allowed to migrate toward vehicle or LPA overnight through Matrigel-coated Transwells. Shown is the average from three independent experiments. Errors are SEM. **, P < 0.01; ***, P < 0.001. (C) Representative Western blot of siRNA-transfected MDA-MB-231 cells.