Fig 8.

RelA antagonizes Hes6-mediated inhibition of Hes1 and promotion of cortical neuronal differentiation. (A) Quantification of luciferase activity in HEK293 cells transfected with a reporter plasmid in which luciferase expression is under the control of an ∼3.0-kb fragment of the neurogenin3 promoter, which contains multiple Hes1 binding sites. Reporter plasmid was transfected alone (luciferase activity is considered 100%) or in combination with plasmids encoding Hes1 in the absence or presence of Hes6 and increasing amounts of RelA (0.5 or 1.0 μg/transfection). Results are shown as the means ± SD (**, P < 0.01; n = 4 separate experiments performed in duplicate; t test). (B) Quantification of the percentage of GFP⁺ cells coexpressing Ki-67, nestin, βIII-tubulin (βIII-tub), or MAP2 72 h after transfection of E13.5 telencephalon-derived cortical progenitor cells using plasmids encoding GFP alone (control) or together with the indicated proteins. Results are shown as the means ± SD (>500 cells were counted in each case; *, P < 0.05; ***, P < 0.001; n = 5 separate experiments performed in duplicate; one-way ANOVA followed by Tukey's post hoc test).