FIG. 2.

Immunostaining of endothelial-specific PECAM1 on CPC with HUVEC+HOB coculture at 42 days. HUVEC were stained green. Both HUVEC and HOB nuclei were stained blue; however, the HOB had blue nuclei with no green staining. (A) HUVEC on macroporous CPC had self-assembled into a microcapillary-like structure with an extensive net-like pattern. (B) Higher magnification showed that the microcapillary-like structures (long arrows) extended throughout the HOB (short arrows). (C) The microcapillary-like structure was highly branched and interconnected. (D) Higher magnification revealed that the diameter of the microcapillary-like tubes consisted of two to three HUVEC, as indicated by the location of nuclei (arrows) and the PECAM1 staining. Color images available online at www.liebertpub.com/tea