Skip to main content
. 2013 Jul;87(14):8004–8016. doi: 10.1128/JVI.00506-13

Fig 6.

Fig 6

PI3K is important for the reactivation of latent KSHV following triggering of the BCR. (A) LY294002 inhibits the reactivation of latent KSHV following triggering of the BCR. BJAB and BrK.219 were treated for 1 h with increasing amounts of inhibitor or vehicle control (DMSO). Next, reactivation was induced by the addition of antibodies against IgM (2.5 μg/ml). After incubation for 3 days, supernatants were titered on HEK 293 cells. The results are represented as the means of duplicate samples ± the standard deviations. (B) Cell lysates of the same experiments (left panel) and their nonreactivated control samples (right panel) were analyzed by SDS-PAGE and immunoblotting with the indicated antibodies. (C) PI-103 inhibits KSHV reactivation induced by treatment with anti-IgM but not with TPA (2 ng/ml). (D) The metabolic activity of BrK.219 cells treated for 3 days with the inhibitor LY294002 (5 or 7.5 μM), PI-103 (0.5 or 0.75 μM) or vehicle control (DMSO) was analyzed by MTT assay. The results are presented as the means of triplicates ± the standard deviations.