Sensitivity of Vero cell/VSV-GFP bioassays for detection of different types of IFN. The recombinant human IFNs IFN-α, IFN-β, IFN-γ, IL-28A, IL-28B, and IL-29 in the concentration range of 0.01 to 100,000 U/ml were applied to Vero cells for 24 h prior to challenge with VSV-GFP. Percentages of GFP-positive cells were determined by flow cytometry 24 h later.