Fig 4.
LMP2A downregulates Bim expression during cell detachment. (A) LMP2A− EBV- and WT EBV-positive cells were cultured in suspension for 40 h, and Bim protein expression was analyzed by Western blotting. β-Actin protein was analyzed as an internal control. (B) Effect of U0126 on Bim induction in LMP2A− EBV- and WT EBV-positive cells. U0126-treated or untreated cells were cultured in suspension for 40 h, and then Bim expression was analyzed. (C) LMP2A-transfected and nontransfected (Neor) cells were cultured in suspension for 40 h, and Bim protein expression was analyzed. β-Actin expression was analyzed as an internal control. (D) Effect of U0126 on Bim induction in LMP2A-transfected and Neor cells. U0126-treated or untreated cells were cultured in suspension for 40 h and were then analyzed for Bim expression.