Fig 2.

Plaque morphology of mutant viruses in comparison to HSV-1(F)-YE102. Confluent monolayers of Vero cells were infected with wild-type and mutant viruses at an MOI of 0.0001 and immunohistochemically stained at 48 hpi using polyclonal anti-HSV rabbit sera as described in Materials and Methods. (A) Representative viral plaques of HSV-1(F)-YE102 wild-type virus and the syncytial mutant viruses gBΔ28 and gKsyn20 on Vero cells. (B) Representative viral plaques of syncytial mutant viruses lacking gM or UL11 in the absence or presence of transient complementation with either gM- or UL11-expressing plasmid.