Characterization of glycoprotein expression by wild-type and mutant viruses. The overall synthesis of each viral protein was assessed by Western immunoblotting. Each lane represents infected cellular extracts from cells infected with either wild-type or mutant virus: 1, wild type; 2, gBΔ28; 3, gBΔ28/ΔgM; 4, gBΔ28/ΔUL11; 5, mock infection; 6, gKsyn20; 7, gKsyn20/ΔgM; 8, gKsyn20/ΔUL11. gB, gC, gD, gM, and UL11 denote antibodies specific for each protein.