Fig 6.

Cell surface expression of viral glycoproteins on infected cell surfaces by wild-type and mutant viruses. At 12 hpi, whole lysates were prepared and membrane-associated proteins were isolated (see Materials and Methods). Flowthrough-labeled lanes represent cytoplasmic proteins. Each lane represents wild-type or mutant virus-infected cell extracts. (A) Lane 1, wild type; lane 2, ΔgM; lane 3, ΔUL11. (B) Lane 1, gBΔ28; lane 2, gBΔ28/ΔgM; lane 3, gBΔ28/ΔUL11. (C) Lane 1, gKsyn20; lane 2, gKsyn20/ΔgM; lane 3, gKsyn20/ΔUL11. gB, gC, gD, gH, gM, UL11, and VP5 denote antibodies specific for each protein.