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Detection of DI-like RNAs in clinical specimens. (A and B) RNA extracted from selected INSIGHT clinical specimens (INS002 to INS036) was subjected to PB2 segment-specific RT-PCR using SuperScript III (SSIII) first-strand synthesis kit MLV RT (Invitrogen) (A) or AMV RT (New England BioLabs) (B). INS036 is a negative control. The positions of molecular size standards (in kilobases) are shown to the left of the gels.
