Fig 2.

CD55 expression increases upon HCV infection of hepatocytes. (A) Enhanced CD55 mRNA expression in IHH infected with HCV genotype 1a and PHH infected with HCV genotype 2a detected by qRT-PCR. Parental hepatocytes were used as a control for comparison. CD55 mRNA expression in parental hepatocytes was arbitrarily set at 1, and the results for the infected hepatocytes were statistically significantly different (P < 0.0001) using one-way ANOVA. (B) Detection of HCV core protein expression in HCV genotype 2a-infected Huh7.5 cells by Western blotting. Cellular actin expression was used as a loading control in each lane. (C) Staining with anti-CD55 antibody from HCV genotype 1a-infected IHH is shown by flow cytometry analysis.