Fig 5.

Tm-1 inhibits complex formation of ToMV replication proteins with host membrane proteins. ToMV RNA derivatives encoding the FLAG-tagged 130K protein of either Tm-1-sensitive (Ile) or resistance-breaking (LT1) type with (130F) or without (130Δ3′) the 3′ UTR (Fig. 4A) were translated in mdBYL, incubated with mdBYL from Tm-1-expressing cells, and then incubated with BYL membranes. The mixtures were fractionated into soluble (S16) and membrane (P16) fractions. The 130K protein in the P16 fraction was solubilized with LPC and immunoprecipitated (IP) with anti-FLAG antibody. The precipitates were analyzed by Western blotting to detect the indicated proteins.