. 2013 Jul;87(14):8038–8052. doi: 10.1128/JVI.03460-12

Table 1.

Sequences of primers used in this study

Clone or mutant	Sense^a	Primer oligonucleotide sequence (5′–3′)
Clones
ORF36-pA3F/pA3 M	S	`TTTGAATTCCGCCATGCGGTGGAAGAATGGAGAG`
	AS	`TTTGCGGCCGCGAAAACAAGTCCGCGGGTGTGGGG`
pLVX-ORF59-Flag	S	`TTTGAATTCTCCTGTGGATTTTCACTATGGGGTC`
	AS	`TCAGCGGCCGCGAAAACAAGTCCGCGGGTGTGGGG`
ORF59-HA-pxi	S	`TTTGAATTCCACCATGCCTGTGGATTTTCACTATGGG`
	AS	`TTTGGATCCTTAGCCCTAAGCGTAATCTGGAACATCGTATGGGTACCAACCCGGGACTTTACACAGTCT`
ORF59-GST	S	`TTTGGATCCATGCCTGTGGATTTTCACTAT`
	AS	`TTTGAATTCGTAGGAAATGGTGGTCCTGA`
ORF59 1-132aa GST	S	`TTTGAATTCCACCATGCCTGTGGATTTTCACTATGGG`
	AS	`TTTGAATTCGTAGGAAATGGTGGTCCTGA`
ORF59 133-264aa GST	S	`TTTGGATCCGGGGACAACCTCACCTCCACC`
	AS	`TTTGAATTCCCAACCCGGGACTTTACACAGTCT`
ORF59 265-396aa GST	S	`TTTGGATCCTTTACCCCCGGGCTGATTTGG`
	AS	`TTTGAATTCGTAGGAAATGGTGGTCCTGA`
Mutants
ORF36-K108Q-pA3F	S	`GTGTGCAGCAGTTTGATAGCCGCCGGG`
	AS	`AACTGCTGCACACACAGATCCTCGGAGA`
ORF59-d304-318 (DM1)	S	`CCGATCTGTGATACCGGACTCCAGGAGGCA`
	AS	`AGTCCGGTATCACAGATCGGTTTACCTCTA`
ORF59-d333-349 (DM2)	S	`GTCTCCGGATGAGGACGCTGCCTCTGTCAC`
	AS	`CAGCGTCCTCATCCGGAGACTCCAATTCCG`
ORF59-d376-379 (DM3)	S	`CAAGAGGCGCCAGTCCAGGGATCGTGGGAA`
	AS	`CCCTGGACTGGCGCCTCTTGTGAGGCCTCT`
ORF59 S305A, S312A, S318A (PM1)		`AACCGATCTGTGGCAGCCGAGGGAGGCGAGTCTGCACAAAAGGTTCCCGATGCAATACCGGACTCC`
ORF59 S333A, S349A (PM2)		`TTGGAGTCTCCGGATGCACCCCCTCTCACACCA`
ORF59 S376A, S378A, S379A (PM3)		`CCTCACAAGAGGCGCGCAGACGCGGCCCAGTCCAGGGATCGT`
ORF59 S376A		`CCTCACAAGAGGCGCGCAGACTCGAGCCAGTCCAGGGATCGT`
ORF59 S378A		`CCTCACAAGAGGCGCTCAGACGCGAGCCAGTCCAGGGATCGT`
ORF59 S379A		`CCTCACAAGAGGCGCTCAGACTCGGCCCAGTCCAGGGATCGT`

S, sense; AS, antisense.