Fig 1.
NKG2Chi CD57hi NK cells are associated with HCMV seropositivity. (A) Freshly isolated PBMCs from HCMV-seropositive (n = 35) and -seronegative (n = 24) healthy donors were stained with PerCP–Cy5.5–anti-CD3 (UCHT1), APC–anti-CD56 (B159), PE–anti-CD57 (NK-1), and AF488-NKG2C (134591) antibodies. After gating on lymphocytes, the percentages of NKG2Chi CD57hi NK cells gated on CD3− CD56dim (left), NKG2Chi CD57hi NKT cells gated on CD3+ CD56+ (middle), and NKG2Chi CD57hi T cells gated on CD3+ CD56− (right) are shown. Statistical significance is indicated at the top (Mann-Whitney U test). The horizontal lines represent the mean for each group. (B) Results of representative four-color staining to identify NKG2Chi CD57hi NK cells from 3 HCMV-seropositive donors and 1 seronegative donor are shown.