Fig 2.

Knockdown of endogenous Tr/E by siRNA significantly increased HSV-2 replication in HEC-1A cells. HEC-1A cells were either left untreated (UT) or transfected with a nontargeting control siRNA (ctrl siRNA) or Tr/E siRNA for 48 to 72 h with subsequent challenge with HSV-2 at an MOI of 0.1 and 1 for 2 h. After repeated washes to remove viral inoculum, cells were cultured for an additional 24 h. Levels of Tr/E in supernatants were measured by ELISA (A), and viral titers were determined by plaque assay (B). The data are representative of at least two independent experiments performed in triplicate and are shown as the means ± SD. Statistical analysis was performed using Student's t test, with significance indicated in the graphs.