Fig 1.

Foamy virus infectivity is correlated to Gag maturation. (A) Schema of the Gag p68-p3 cleavage sites used in this study. All plasmids were constructed by site-directed mutagenesis. wt, wild type. (B) Western blotting of purified recombinant with a monoclonal anti-Gag (upper and middle panel) or anti-Pol antibodies (lower panel). Positions of the size markers are indicated. WT, wild type. (C) Viral titers were determined on BHK-21 cells by serial dilutions as described before (8, 9). Error bars indicate the standard deviations of triplicate samples.