Fig 1.

Interaction of DYRK1A with HPV21 E6 protein. (A) Homology between the C-terminal region of E1A and the N-terminal regions of 21/14E6. The E1A deletion mutants that are defective in interaction with different cellular protein complexes are indicated. E1A mutants dl1133 and dl1134 are defective in interaction with the DYRK1/HAN11 complex. (B) Mutants of 21E6. (C) Immunoprecipitation analysis of DYRK1A interaction with 21E6. The E6-GFP-Flag fusion proteins of wt 21E6 or E6 mutants (codon optimized) were transiently expressed in 293 cells using the Tet-GFP-inducible vector and immunoprecipitated with the Flag antibody (Ab) as described previously (11). The Western blots were probed with Abs specific to DYRK1A, FOXK1, or the Flag epitope.