Fig 3.

Interaction of DYRK1A with 5E6 and 8E6 variants. (A) N-terminal amino acid sequences of 5E6 and 8E6 variants. The amino acids that are different from the prototype E6 proteins are highlighted in black. The bipartite motif implicated in DYRK1/HAN11 interaction of 21E6 and the homologous sequences in 5E6 and 8E6 are indicated by grey shading. (B) Interaction of endogenous DYRK1A. The interaction of endogenous DYRK1A with Flag-tagged E6-GFP fusion proteins of 5E6 and 8E6 was determined as for Fig. 1. The variants deficient in DYRK1A interaction are circled. (C) Interaction of GFP-DYRK1A with E6 variants. The interaction of E6 variants with exogenously introduced GFP-DYRK1A was determined as for Fig. 2.